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Back-Illuminated sCMOS with Large Pixels

Prime 95B

The Prime 95B was the first back-illuminated sCMOS camera, and offers extreme sensitivity thanks to 95% QE, large pixels and low noise. The Prime 95B also operates at high frame rates across a large sensor, consistently outperforming EMCCD cameras.

Prime 95B
 

Sensitivity

The Prime 95B back-illuminated sCMOS camera achieves a near-perfect 95% quantum efficiency.

By bringing the light in from the back of the sensor, photons land directly onto the light receiving surface, maximizing light collecting capability. Combining this with large, 11 µm pixels results in a camera technology as sensitive as EMCCD with greater stability, faster speeds and no gain aging.

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Superior Background Quality

The Prime 95B features Pattern Noise Reduction Technology and Correlated Noise Reduction Technology to ensure that it delivers clean, pattern-free images with minimal pixel defects, delivering improved image quality in low light conditions.

 

Multiple Fields of View

The Prime 95B is available with an 18.8, 22 or 25 mm diagonal field of view. The larger format sensors are designed to increase throughput, maximize the amount of data captured and take full advantage of new, larger field of view microscopes.

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Advanced Features

The Prime 95B offers two data reduction technologies; PrimeLocate and Multi-ROI.

PrimeLocate can also be extended to offer Live Particle Tracking on the camera, giving early insight into the kinetics of moving molecules for single molecule tracking applications.

PrimeEnhance active denoising provides a real-time quantitative increase in signal to noise ratio by reducing the effects of photon shot noise at low light levels.

 

Advanced Triggering

Sequenced Multiple Acquisition Real Time (SMART) Streaming enables the Prime 95B to capture a continuous sequence of images while cycling through 16 pre-programmed exposure time values, delivering the fastest multi-channel acquisition times.

The Prime 95B also supports up to four expose-out signals and has the capability for an effective global shutter.

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Family Specifications

Sensor Technology sCMOS
Quantum Efficiency 95%
Pixel Size 11 µm x 11 µm
Supported Interfaces PCIe and USB 3
Spectrum Capabilities UV (200-440 nm), Visible (400-700 nm), Near Infrared (700-1000 nm)
Read Noise (median) 1.6 e-
 

Model Finder

FOV (Diag. mm) MP FPS Pixel Size (μm) QE Read Noise Sensor Status
Prime 95B
01-PRIME-95B-R-M-16-C
13.3 x 13.3 mm 1.4 80 fps 11 µm 95% 1.6 e⁻ GPixel Gsense 400 BSI In Production Request Info Prime 95B
Prime 95B (Plus Features)
01-PRIME-95B-R-M-16-C-ENH-LOC
13.3 x 13.3 mm 1.4 80 fps 11 µm 95% 1.6 e⁻ GPixel Gsense 400 BSI In Production Request Info Prime 95B
Prime 95B 22mm
01-PRIME-95B-22MM-R-M-16-C
15.5 x 15.5 mm 1.9 68 fps 11 µm 95% 1.6 e⁻ GPixel Gsense 400 BSI In Production Request Info Prime 95B
Prime 95B 25mm
01-PRIME-95B-25MM-R-M-16-C
17.7 x 17.7 mm 2.5 60 fps 11 µm 95% 1.6 e⁻ GPixel Gsense 400 BSI In Production Request Info Prime 95B

Resources & Support

Tech Note

Prime 95B sCMOS vs EMCCD For Super-Resolution Imaging

Customer Story

Single Molecule Biophysics

Customer Story

Single Molecule Detection

Customer Story

Single Molecule FRET

Customer Story

Single Molecule Imaging at University of Lund

Customer Story

Single Molecule Imaging at University of York

Customer Story

Single Molecule Imaging at University of Zürich

Customer Story

Single Molecule Spectroscopy at Heidelberg University

Customer Story

Single Molecule TIRF

Customer Story

Single Molecule Tracking PALM

Below are assets shared by all models of this product family.

For model-specific assets, select an option in the Model Finder above.

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PVCAM SDK & Driver

Software Download

Beacon

Software Download

Ocular 2.0

Software Download

Version: 2.0

3rd Party Software for Photometrics cameras

Software Download

Below are assets shared by all models of this product family.

For model-specific assets, select an option in the Model Finder above.

Prime 95B Customer Applications Handbook

Brochure

Prime 95B Datasheet

Datasheet

Last Updated: March 23, 2026

Prime 95B Family Manual

User Manual

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