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Back-Illuminated sCMOS with Low Read Noise

Prime BSI

The Prime BSI sCMOS is a back-illuminated camera designed to provide a balance between high resolution imaging and sensitivity, with the combination of a 6.5 µm pixel and near-perfect 95% quantum efficiency making it an ideal solution for a range of applications. Also featuring CMS mode for ultra-low read noise of 1.0 electrons!

 

Sensitivity

The Prime BSI back-illuminated sCMOS camera achieves a near-perfect 95% quantum efficiency. By bringing the light in from the back of the sensor, photons land directly onto the light receiving surface, maximizing light collecting capability. The Prime BSI combines 95% quantum efficiency with a low 1.0 e- read noise to deliver the most sensitive camera based on sCMOS technology at over 40 frames per second.

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Resolution and Pixel Size

The Prime BSI features 6.5 μm pixels, the accepted standard for most live cell applications using 40x and 60x magnification. This pixel size provides highly detailed images across the imaging plane and is most suitable for the broadest range of microscope objectives.

 

Superior Background Quality

The Prime BSI features Pattern Noise Reduction Technology and Correlated Noise Reduction Technology to ensure that it delivers clean, pattern free images with minimal pixel defects, delivering improved image quality in low light conditions.

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Advanced Triggering

Programmable Scan Mode provides increased control over the rolling shutter exposure and read-out functionality of CMOS sensors for applications that require control over the line time.

Sequenced Multiple Acquisition Real Time Streaming (SMART) Streaming enables the Prime BSI to capture a continuous sequence of images while cycling through 16 pre-programmed exposure time values, delivering the fastest multi-channel acquisition times.

 

Advanced Features

The Prime BSI offers PrimeEnhance active denoising which provides a real-time quantitative increase in the signal to noise ratio to maximize sensitivity at ultra-low light levels.

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Family Specifications

Sensor Technology sCMOS
Quantum Efficiency 95%
Pixel Size 6.5 µm x 6.5 µm
Supported Interfaces PCIe and USB 3
Spectrum Capabilities UV (200-440 nm), Visible (400-700 nm), Near Infrared (700-1000 nm)
Read Noise (median) 1.0 e-
 

Model Finder

Product MP FPS Pixel Size (μm) QE Read Noise Sensor Price Compare Options
Prime BSI (PCIe)
01-PRIME-BSI-R-M-16-C-PCIE
4.1 63 fps 6.5 µm 95% 1.0 e⁻ GPixel Gsense 2020BSI In Production Request Info Prime BSI
Prime BSI (USB 3.0)
01-PRIME-BSI-R-M-16-C
4.1 63 fps 6.5 µm 95% 1.0 e⁻ GPixel Gsense 2020BSI In Production Request Info Prime BSI

Media gallery

Setting up a Prime BSI Express in Olympus CellSens Software
Setting up a Prime BSI Express in Olympus CellSens Software

Resources & Support

System Requirements

Recommended PC Specifications

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Customer Story

High Content Multiplex Fluorescence at University Hospital Bonn

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Customer Story

Calcium Imaging at Medical University of Vienna

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Customer Story

Cryo Super-Resolution Imaging

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Customer Story

eSPIM Light Sheet

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Customer Story

High Content Multiplex Fluorescence at Institute of Science and Technology Austria

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Customer Story

Imaging Mitotic Dynamics

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Customer Story

Imaging With A Quantum Light Source

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Customer Story

Light Sheet Optical Thermometry

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Customer Story

Liquid Beam Observation and Fluorescence

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Below are assets shared by all models of this product family.

For model-specific assets, select an option in the Model Finder above.

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PVCAM Software 3.10.1.1

Software Download

Version: 3.10.1.1

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PVCAM 3.10.0.3 (Linux)

Software Download

Version: 3.10.0.3

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PVCAM 3.8.0.6

Software Download

Version: 3.8.0.6

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3rd Party: Dotphoton Jetraw Raw Image Data Compression Tool

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3rd Party: Intelligent Imaging Innovations (3i) Slidebook 6 Software

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3rd Party: MathWorks MATLAB Image Acquisition Toolbox

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3rd Party: Micro-Manager Open Source Microscopy Software

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3rd Party: Molecular Devices MetaMorph

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3rd Party: National Instruments LabVIEW System Design Software

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3rd Party: Nikon NIS-Elements Software

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Below are assets shared by all models of this product family.

For model-specific assets, select an option in the Model Finder above.

Prime BSI Customer Applications Handbook

Brochure

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Prime BSI Datasheet

Datasheet

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Prime BSI Manual

User Manual

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